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KMID : 1134820080370050612
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 5 p.612 ~ p.617
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Toxin Profile in the Liver of Puffer Fish, Takifugu niphobles, and Changes in Mouse Toxicity by pH and Heating Conditions
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Jang Jun-Ho
Yun So-Mi
Kim Jung-Soo
Lee Jong-Soo
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Abstract
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Tetrodotoxin (TTX) analogues were first determined from the liver extracts of puffer fish, Takifugu niphobles, by LC/MS with Hydrophilic Interaction Liquid Chromatography (HILIC). In total, 7 TTX analogues were detected within 20 minutes as follows; 5,6,11-trideoxyTTX (34.0%, 1,029.6 nmol/g), 6,11-dideoxyTTX (29.3%, 887.6 nmol/g), TTX (22.1%, 667.8 nmol/g), 4,9-anhydro-TTX (11.2%, 339.3 nmol/g), 11-deoxyTTX ＋5-deoxyTTX (2.6%, 78.6 nmol/g), and 4-epiTTX (0.8%, 23.6 nmol/g). Mouse toxicity of diluted liver extracts showed the highest toxicity at pH 3 (8.7 MU/mL) and decreased, as increasing pH, to 1.4 MU/mL at pH 10. At acidic (pH 5) and neutral conditions (pH 7), mouse toxicity of liver extracts (79 MU/mL) decreased slowly, as increasing temperature from 80oC to 115oC, and time until 1 hour; in contrast, at the akaline condition (pH 9), the toxicity decreased rapidly to the more than half within 10 minutes. Individual toxicity of the fillet of
T. niphobles were between 43.2～106.7 MU, and 64～78% of its toxicity was eluted to soup when boiled with 3 volumes of water during 10 minutes.
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KEYWORD
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tetrodotoxin (TTX) analogues, puffer fish, Takifugu niphobles, LC/MS, mouse toxicity
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